Dallas, D. C., A. Guerrero, N. Khaldi, P. A. Castillo, W. F. Martin, J. T. Smilowitz, C. L. Bevins, D. Barile, J. B. German and C. B. Lebrilla (2013). “Extensive in vivo Human Milk Peptidomics Reveals Specific Proteolysis Yielding Protective Antimicrobial Peptides.” J Proteome Res 12(5): 2295-2304. Milk is traditionally considered an ideal source of […]

Jincui Huang; Hyeyoung Lee; Angela Zivkovic; Jennifer Smilowitz; Bruce German; Carlito Lebrilla UC Davis, Davis, CA NOVEL ASPECT: Extensive site-specific glycosylation of HDL proteins and glycolipid analysis indicate that HDL is a highly glycosylated and sialylated particle. INTRODUCTION: High-density lipoprotein (HDL) particles are composed of lipids and lipoproteins and are considered as a strong inverse predictor of risk […]

Serenus Hua1; Lauren Dimapasoc2; Mary Saunders²; Bum Jin Kim1; Seung Hyup Jeong1; Kit S. Lam², Carlito Lebrilla2; Hyun Joo An1 1GRAST, Chungnam National University, Daejeon, SOUTH KOREA; 2University of California, Davis, Davis, CA NOVEL ASPECT: Stark differences in cancer cell membrane glycosylation can be exploited to create an MS-based biopsy INTRODUCTION: In clinical settings, biopsies are routinely used to determine cancer type […]

David Dallas1; Andres Guerrero1; Nora Khaldi2; Bruce German1; Daniela Barile1; Mark Underwood1; Carlito Lebrilla1 1University of California, Davis, Davis, CA; 2UC Dublin, Dublin, Ireland NOVEL ASPECT: This study represents a novel application of high-throughput peptidomics to investigate milk protein digestion in term and premature infants. INTRODUCTION: Decades of research demonstrate that human milk protein digestion releases peptide fragments with beneficial […]

Sarah Totten; Santiago Ruiz-Moyano; David Mills; Carlito Lebrilla University of California Davis, Davis, CA NOVEL ASPECT: The application of mass spectrometry based glycan arrays and high-throughput HMO analysis to characterizing the specificity of glycosidases in bacteria INTRODUCTION: Human milk oligosaccharides function as prebiotics for beneficial bacteria that occupy the gut of breast fed infants. Certain species of Bifidobacteria are equipped with the […]

Evan Parker; Qiuting Hong; Andres Guerrero; Michael Xin Sun; Jincui Huang; Carlito Lebrilla UC Davis, Davis, CA NOVEL ASPECT: Direct comparison of trypsin and nonspecific methods using new software for analyzing glycopeptides with an improved scoring algorithm. INTRODUCTION: The determination of site-specific glycosylation in proteins remains a difficult task.  Two methods currently in use employ specific proteases such as trypsin or […]

Nora Khaldi1, 2; Vaishnavi Vijayakumar1; Andreś Guerrero2; Norman Davey3; Carlito Lebrilla2; Denis Shields1 1University College Dublin, Dublin, IRELAND; 2University college Davis, Davis, USA; 3European Molecular Biology Laboratory, Heidelberg, Germany NOVEL ASPECT: This tool will greatly advance the understanding of the mechanisms of action of enzymes, and the prediction of bioactive peptides. INTRODUCTION: For many years, MS has been widely employed […]

Ting Song; Danielle Aldredge; Javier González; Carlito Lebrilla University of California Davis, Davis, California NOVEL ASPECT: We constructed a serum-based library containing 164 structures with relative abundances, retention times, accurate masses, and tandem MS spectra. INTRODUCTION: Glycosylation is an abundant and common posttranslational modification of proteins with functions related to stabilization of protein conformation, secretion through membranes, protein turnover control, proteinase […]