Qiuting Hong1; L. Renee Ruhaak1; Suzanne Miyamoto2; Carlito Lebrilla1 1Chemsitry, UC, Davis, Davis, CA; 2Comprehensive Cancer Center, UC, Davis, Davis, CA NOVEL ASPECT: Monitoring the degree of glycosylation with the absolute protein quantification using MRM methods INTRODUCTION: The studies aimed towards glycan biomarker discovery have focused on glycan characterization and profiling of released glycans. Site-specific glycosylation analysis is less […]

Mass Spectrometry of Glycans and Glycoproteins Jon Amster (Universty of Georgia), Carlito Lebrilla (University of California, Davis), Ron Orlando (University of Georgia), Joe Zaia (Boston University) This course is designed for scientists who want to learn specific techniques for the MS and MS/MS characterization of glycans and glycoproteins. The course will address fundamental aspects of glycobiology, sample preparation […]

Andres Guerrero1; Dave C. Dallas2; Nora Khaldi3; Daniela Barile2; J. Bruce German2; Carlito B. Lebrilla1 1UC Davis, Chemistry Department, Davis, CA; 2UC Davis, Food Science Department, Davis, CA; 3Food for Health Ireland, University College Dublin, Belfield, Ireland NOVEL ASPECT: A comprehensive MS method was used to analyze endogenous peptides from human milk that were shown to be antimicrobial. INTRODUCTION: A novel, […]

Lauren M. Dimapasoc; Sarah Totten; Carol Stroble; L. Renee Ruhaak; Carlito B. Lebrilla University of California, Davis, CA NOVEL ASPECT: HMO extraction and purification at the 96-well level now allows for large-scale biomarker studies. INTRODUCTION: Human milk is known to be the best nutritional source for newborns. Human milk oligosaccharides (HMOs) are a highly abundant constituent in human milk, […]