Authors Carlito Lebrilla; Evan Parker; Michael Xin Sun; Jincui Huang; Andres Guerrero Institutes UC Davis, Davis, CA Novel Aspect Software allows the use of any enzymatic system for the analysis of glycopeptides by LCMS thereby allowing target-oriented glycopeptide analysis. Introduction Site-specific analysis of protein glycosylation is an active and evolving field of research. Successful application […]

Authors Lauren D. Wu; Angela Zivkovic; Sarah Totten; L. Renee Ruhaak; Carlito B. Lebrilla Institutes University of California, Davis, CA Novel Aspect The stability of oligosaccharides in human breast milk during sample handling and storage is assessed for the first time. Introduction Human milk oligosaccharides (HMOs) are one of the more abundant bioactives in human […]

Authors Renee Ruhaak1; Carol Stroble1, 2; Qiuting Hong1; Suzanne Miyamoto2; Kyoungmi Kim1; Gary Leiserowitz2; Carlito Lebrilla1 Institutes 1UC Davis, Davis, CA; 2UC Davis Medical Center, Sacramento, CA Novel Aspect A QQQ-MS approach is presented for the discovery and validation of serum protein and glycopeptide levels as discriminators of disease. Introduction Protein glycosylation has been proposed as […]

Authors Jincui Huang; Qiuting Hong; Rocchina Sabia; Carlito Lebrilla Institutes UC Davis, Davis, CA Novel Aspect Rapid-throughput method of quantitation of human milk proteins and their glycoforms was developed. Introduction Human milk has long been recognized as not only a source of nutrition but for a number of functional components to the newborn. Milk proteins […]

Authors Qiuting Hong; Evan Parker; Ting Song; Carlito Lebrilla Institutes Chemsitry, UC, Davis, Davis, CA Novel Aspect A comprehensive site-specific glycan map for plasma glycoproteins Introduction Glycosylation analysis on the site-specific level provides both protein and glycosylation information to facilitate biomarker discovery. However, site-specific analysis remains a difficult task, and there is no comprehensive glycan […]

Authors Jasmine C. C. Davis; Sarah M. Totten; Carlito B. Lebrilla Institutes UC Davis, Davis, CA Novel Aspect This research aimed to create a library of intact and degraded milk glycans found in infant feces from microbial catabolism. Introduction Oligosaccharides continue to be one of the most difficult biomolecules to analyze. Analysis is, however, aided […]

Authors Evan Parker; Carlito Lebrilla Institutes UC Davis, Davis, CA Novel Aspect First application of monodisperse polymers for encoding information in materials. Derivative methods could lead to commodity utility of mass spectrometry. Introduction Product safety of pharmaceuticals and food products being of paramount, industry expends great effort in the tracking and validation of product source […]