TWO-DAY COURSE, Saturday and Sunday
Glycans and Glycoproteins for Mass Spectrometry


Jon Amster
University of Georgia
Carlito Lebrilla
University of California, Davis
Ron Orlando
University of Georgia
Joe Zaia
Boston University

This course is designed for scientists who want to learn specific techniques for the MS and MS/MS characterization of glycans and glycoproteins. The course will address fundamental aspects of glycobiology, sample preparation and handling, mass spectrometry (hardware and software), and bioinformatic tools for interpretation of results.

Real-world examples of the application of these techniques will include characterization of intact glycoproteins, characterization of released glycans, analysis of complex mixtures of glycoproteins and glycans. The role of MS-based methods in interdisciplinary efforts to solve these complex problems will also be addressed.

Day 1
 Introduction to glycosylation
 Chemical manipulation/derivatization of oligosaccharides, GC-MS for linkage and composition analyses
 MALDI MS and ESI of oligosaccharides
 Tandem MS of oligosaccharides
 Glycoproteins — release and analyze, and glycomic approaches
 Glycopeptides, methods for site specific glycosylation analysis, glycoproteomic approaches

Day 2
 Glycoprotein applications and case studies
 Glycosaminoglycans: Background, fundamentals, Tandem MS
 Glycolipids
 Glycan Quantitation
 Bioinformatics

Pre-requisites: A basic knowledge of mass spectrometry and some rudimentary knowledge of biology
and chemistry is desirable.