David Dallas1; Andres Guerrero1; Nora Khaldi2; Bruce German1; Daniela Barile1; Mark Underwood1; Carlito Lebrilla1 1University of California, Davis, Davis, CA; 2UC Dublin, Dublin, Ireland NOVEL ASPECT: This study represents a novel application of high-throughput peptidomics to investigate milk protein digestion in term and premature infants. INTRODUCTION: Decades of research demonstrate that human milk protein digestion releases peptide fragments with beneficial […]

Sarah Totten; Santiago Ruiz-Moyano; David Mills; Carlito Lebrilla University of California Davis, Davis, CA NOVEL ASPECT: The application of mass spectrometry based glycan arrays and high-throughput HMO analysis to characterizing the specificity of glycosidases in bacteria INTRODUCTION: Human milk oligosaccharides function as prebiotics for beneficial bacteria that occupy the gut of breast fed infants. Certain species of Bifidobacteria are equipped with the […]

Evan Parker; Qiuting Hong; Andres Guerrero; Michael Xin Sun; Jincui Huang; Carlito Lebrilla UC Davis, Davis, CA NOVEL ASPECT: Direct comparison of trypsin and nonspecific methods using new software for analyzing glycopeptides with an improved scoring algorithm. INTRODUCTION: The determination of site-specific glycosylation in proteins remains a difficult task.  Two methods currently in use employ specific proteases such as trypsin or […]

Nora Khaldi1, 2; Vaishnavi Vijayakumar1; Andreś Guerrero2; Norman Davey3; Carlito Lebrilla2; Denis Shields1 1University College Dublin, Dublin, IRELAND; 2University college Davis, Davis, USA; 3European Molecular Biology Laboratory, Heidelberg, Germany NOVEL ASPECT: This tool will greatly advance the understanding of the mechanisms of action of enzymes, and the prediction of bioactive peptides. INTRODUCTION: For many years, MS has been widely employed […]

Ting Song; Danielle Aldredge; Javier González; Carlito Lebrilla University of California Davis, Davis, California NOVEL ASPECT: We constructed a serum-based library containing 164 structures with relative abundances, retention times, accurate masses, and tandem MS spectra. INTRODUCTION: Glycosylation is an abundant and common posttranslational modification of proteins with functions related to stabilization of protein conformation, secretion through membranes, protein turnover control, proteinase […]

Qiuting Hong1; L. Renee Ruhaak1; Suzanne Miyamoto2; Carlito Lebrilla1 1Chemsitry, UC, Davis, Davis, CA; 2Comprehensive Cancer Center, UC, Davis, Davis, CA NOVEL ASPECT: Monitoring the degree of glycosylation with the absolute protein quantification using MRM methods INTRODUCTION: The studies aimed towards glycan biomarker discovery have focused on glycan characterization and profiling of released glycans. Site-specific glycosylation analysis is less […]

Mass Spectrometry of Glycans and Glycoproteins Jon Amster (Universty of Georgia), Carlito Lebrilla (University of California, Davis), Ron Orlando (University of Georgia), Joe Zaia (Boston University) This course is designed for scientists who want to learn specific techniques for the MS and MS/MS characterization of glycans and glycoproteins. The course will address fundamental aspects of glycobiology, sample preparation […]

Andres Guerrero1; Dave C. Dallas2; Nora Khaldi3; Daniela Barile2; J. Bruce German2; Carlito B. Lebrilla1 1UC Davis, Chemistry Department, Davis, CA; 2UC Davis, Food Science Department, Davis, CA; 3Food for Health Ireland, University College Dublin, Belfield, Ireland NOVEL ASPECT: A comprehensive MS method was used to analyze endogenous peptides from human milk that were shown to be antimicrobial. INTRODUCTION: A novel, […]